Microwave fixation of water-cooled insect tissues for immunohistochemistry.

摘要: In this paper a new technique for microwave-accelerated tissue fixation and washing is described. The temperature of the irradiated specimens controlled by means specially designed water-cooling device. Two types fluids, one them containing rather concentrated picric acid procedure were tested empirically on dissected nervous Colorado potato beetle,Leptinotarsa decemlineata (Say). Entire beetles processed in similar way. It was shown that could be as long required good results, without accumulation excessive heat. No differences morphology immunoreactivity observed when compared with standard immersion-fixed controls. A considerable reduction processing time achieved.