Isolation and characterization of TH3, a germ cell-specific variant of histone 3 in rat testis.
作者： P K Trostle-Weige , M L Meistrich , W A Brock , K Nishioka
DOI: 10.1016/S0021-9258(17)47220-6
关键词:
摘要: We have identified and purified TH3, a germ cell-specific histone. It has been characterized by amino acid analysis, tryptic peptide mapping, labeling with cystine, electrophoretic mobility as variant of H3. On fully reduced Triton/acid/urea gels its is retarded more than that the somatic variants H3.2 H3.3, but less H3.1; it migrates between H2As H1s. Germinal cells from adult sexually immature testes were centrifugal elutriation followed Percoll density gradient separation in order to study distribution synthesis TH3. TH3 found significant levels spermatogonia similar or slightly higher amounts spermatocytes round spermatids. The takes place not spermatocytes, contrast other testis-specific histones, TH2A, H1t, TH2B. Therefore, may different role spermatogenesis do histone variants.
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