Microbial and Metabolic Succession on Common Building Materials Under High Humidity Conditions

摘要: Despite considerable efforts to characterize the ecology of bacteria and fungi in built environment (BE), metabolic mechanisms underpinning their colonization successional dynamics remain unclear. Here, we applied bacterial/viral particle counting, qPCR, 16S ITS rRNA amplicon sequencing, metabolomics longitudinally ecological four commonly used building materials maintained at high humidity conditions (~94% RH). We varied natural inoculum provided each material by placing them different occupied spaces, wet surface half samples simulate a flooding event. As expected, showed bacterial viral abundance, with having higher growth rates lower alpha diversity compared non-wetted materials. Wetting described majority variance bacterial, fungal metabolite structure, type only influenced diversity, while location inoculation was weakly associated beta diversity. Metabolites indicative microbial activity were identified, as those that native material. Glucose-phosphate abundant on all (except mold-free gypsum) correlated Enterobacteriaceae, which could indicate potential nutrient source. A compound consistent scopoletin, plant antimicrobial activity, significantly negatively Bacillus positively Pseudomonas enriched medium density fiberboard (MDF) In samples, alkaloids nigragillin fumigaclavine C, both properties, phylum Ascomycota. Nigragillin, also abundance. Thiabendazole azoxystrobin (anti-fungal compounds) highly mold-resistant gypsum wallboard likely directly decreased observed this The significant increase well decrease result reduced growth. Penicillium taxa thiabendazole, suggested persistence resistant strains. Also, specific abundance azoxystrobin, suggesting bi-directional competitive adaptation, metabolites known interfere biofilm formation, explain anti-correlation between these taxa. moisture enabled faster inoculating microorganisms, whose composition, chemistry, competition shaped material, need be considered when determining impact dampness environments.