Levels of glycerate 2,3-P2, 2,3-bisphosphoglycerate synthase and 2,3-bisphosphoglycerate phosphatase activities in rat tissues. A method to quantify blood contamination of tissue extracts

作者: Albert Tauler , Joan Gil , Ramon Bartrons , José Carreras

DOI: 10.1016/0305-0491(87)90167-2

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摘要: Abstract 1. The levels of glycerate 2,3-P2 and 2,3-bisphosphoglycerate synthase phosphatase activities have been determined in isolated rat hepatocytes adipocytes perfused tissues to discard blood contamination. 2. values obtained are much lower than those previously reported, ranging 0.50–40 nmol/g tissue. 3. No relationship appears exist between concentration the enzymatic involved metabolism. 4. Assay tissue extracts constitute a very useful way quantify

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