Hizikia fusiformis fractions successfully improve atopic dermatitis indices in anti-CD3-stimulated splenocytes and 2,4-dinitrochlorobenzene-treated BALB/c mice.

摘要: Objectives In the present study, we aimed to examine whether fractions from an edible sea weed, Hizikia fusiformis, had immunomodulatory effects, particularly anti-atopic effect, by attenuating expression of T cell-dependent cytokines using in-vitro and in-vivo animal atopic dermatitis-like models. Methods The activities were examined in vitro, a 2,4-dinitrochlorobenzene (DNCB)-induced mouse model quantitative real-time polymerase chain reaction, electrophoretic-mobility shift histophathological analysis. Key findings Our results showed that final fraction (F2′) H. fusiformis contained higher amount butanoic acid which was not found other fractions, effectively inhibited cell activation inhibiting dephosphorylation nuclear factor activated cells assay. As consequence, helper cytokines, such as interleukin-2, -4 interferon-γ, significantly while with anti-CD3 antibody. We also skin challenged DNCB successfully recovered when treated 2.5 mg/kg, comparable 0.25% prednicarbate. These indicate F2′ may contribute inhibit eliminating Th cytokines. Conclusions Taken together, concluded containing be new functional candidate, probably acts through inactivation mechanisms.