Identification of a tRNA isopentenyltransferase gene from Arabidopsis thaliana

作者: Anna Golovko , Folke Sitbon , Elisabeth Tillberg , Björn Nicander

DOI: 10.1023/A:1014958816241

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摘要: The tRNA of most organisms contain modified adenines called cytokinins. Situated next to the anticodon, they have been shown influence translational fidelity and efficiency. enzyme that synthesizes cytokinins on pre-tRNA, isopentenyltransferase (EC 2.5.1.8), has studied in micro-organisms like Escherichia coli SaccharomYces cerevisiae, corresponding genes cloned. We here report first cloning functional characterization a homologous gene from plant, Arabidopsis thaliana. Expression S. cerevisiae showed can complement anti-suppressor phenotype mutant lacks MOD5, intrinsic gene. This was accompanied by reintroduction isopentenyladenosine tRNA. is constitutively expressed seedling tissues.

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