Influence of chitosan, acetic acid and citric acid on growth and tropane alkaloid production in transformed roots of Brugmansia candida Effect of medium pH and growth phase

作者: Sandra I. Pitta-Alvarez , Ana M. Giulietti

DOI: 10.1023/A:1006359429830

关键词:

摘要: The effects of chitosan, acetic acid and citric on production release hyoscyamine scopolamine in hairy root cultures Brugmansia candida were studied. Chitosan tested at different concentrations also media pH values. At 5.5, certain concentrations, chitosan increased the content hyoscyamine, promoted both alkaloids. Lowering to 3.5 4.5 reduced accumulation alkaloids roots, but a 4.5, their significantly. Acetic stimulated hyoscyamine.

参考文章(26)
M.D. Brownleader, J.B. Harborne, P.M. Dey, Carbohydrate Metabolism: Primary Metabolism of Monosaccharides Plant Biochemistry. pp. 111- 141 ,(1997) , 10.1016/B978-012214674-9/50004-7
Michael G. Hahn, OLIGOSACCHARIDE ELICITORS AND ELICITOR RECEPTORS Springer Netherlands. pp. 37- 58 ,(1995) , 10.1007/978-94-011-0307-7_5
John L Harwood, Plant Lipid Metabolism Plant Biochemistry. pp. 237- 272 ,(1997) , 10.1016/B978-012214674-9/50007-2
Gregorio Godoy-Hern�ndez, V�ctorM. Loyola-Vargas, Effect of fungal homogenate, enzyme inhibitors and osmotic stress on alkaloid content of Catharanthus roseus cell suspension cultures. Plant Cell Reports. ,vol. 10, pp. 537- 540 ,(1991) , 10.1007/BF00232506
J. Bohlmann, U. Eilert, Elicitor induced secondary metabolism in Ruta graveolens L. Role of chorismate utilizing enzymes. Plant Cell Tissue and Organ Culture. ,vol. 38, pp. 189- 198 ,(1994) , 10.1007/BF00033877
Y. Yamada, D.-J. Yun, T. Hashimoto, Genetic Engineering of Medicinal Plants for Tropane Alkaloid Production Advances in Plant Biotechnology. pp. 83- 93 ,(1994) , 10.1016/B978-0-444-89939-2.50011-5
T. Kanegae, H. Kajiya, Y. Amano, T. Hashimoto, Y. Yamada, Species-dependent expression of the hyoscyamine 6 beta-hydroxylase gene in the pericycle. Plant Physiology. ,vol. 105, pp. 483- 490 ,(1994) , 10.1104/PP.105.2.483
Takashi Hashimoto, Dae-Jin Yun, Yasuyuki Yamada, Production of tropane alkaloids in genetically engineered root cultures Phytochemistry. ,vol. 32, pp. 713- 718 ,(1993) , 10.1016/S0031-9422(00)95159-8
L. A. Sáenz-Carbonell, I. E. Maldonado-Mendoza, O. Moreno-Valenzula, R. Clau-Uitz, M. López-Meyer, C. Oropeza, V. M. Loyola-Vargas, Effect of the medium pH on the release of secondary metabolites from roots of Datura stramonium, Catharanthus roseus , and Tagetes patula cultured in vitro Applied Biochemistry and Biotechnology. ,vol. 38, pp. 257- 267 ,(1993) , 10.1007/BF02916405