Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes

摘要: A number of examples circular plasmids with specific functions are known in both prokaryotes and eukaryotes. Several linear have also been identified, but these all relatively small: large cannot be separated from chromosomal DNA by conventional techniques. There several cases where the genetic evidence suggests that a character is encoded plasmid no can physically detected. This has case for antibiotic synthesis genes Streptomyces; particular SCP1 Streptomyces coelicolor shown to involved methylenomycin production evidence. We report here application orthogonal-field-alternation gel electrophoresis isolation Streptomyces. discovered around 520 kilobases lasaliensis subsequently similar giant other strains. confirmed biosynthesis located on series S. coelicolor. These observations may bear variability unstable species.