Preparation and properties of purified antilactic dehydrogenase.

摘要: Summary Antibodies inhibitory to rabbit muscle lactic dehydrogenase (LD) have been produced in roosters and purified. A reliable assay procedure for anti-LD activity has devised a unit of defined. In these experiments the route injection (intravenous or subcutaneous + intravenous) did not appear influence titer obtained. The peak occurred sooner than precipitin activity. purified antienzyme was found serve as noncompetitive inhibitor dehydrogenase. plot logarithm anti-rabbit LD concentration vs. per cent inhibition resulted sigmoid curve, showing that use large concentrations could approach but never attain 100% inhibition. study kinetics suggested one molecule sufficient inhibit LD. An increased lower with fixed quantity anti-LD. obtained independent temperature. It also apparently order which reagents were added an excessively long time interval required equilibration when coenzyme (DPNH) mixed enzyme before addition antienzyme. Purified antirabbit showed various degrees dehydrogenases from diverse species. general considerable cross reaction mouse