[1] Advanced 5′-silyl-2′-orthoester approach to RNA oligonucleotide synthesis
作者： Stephen A. Scaringe
DOI: 10.1016/S0076-6879(00)17003-X
关键词:
摘要: Publisher Summary This chapter describes the synthesis of RNA oligonucleotide using 5´-silyl-2´-orthoester approach. For some applications, is sufficient purity to use without further processing. After an oligonucleotide, 2´-orthoester protected water soluble and significantly more stable for degradation than final fully deprotected product. These features 2´- orthoester group enable be easily handled in aqueous solutions. The groups interrupt secondary structure. property has made it possible analyze purify oligonucleotides every sequence regardless includes 10 15 base-long homopolymers guanosine. Finally, when ready use, are completely removed less 30 minutes under extremely mild conditions common buffers. unique properties 5´-silyl ether protecting have routinely synthesize high-quality oligonucleotides.
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