Purification of a Fe-SOD excreted by Leishmania braziliensis for specific antibodies detection in Mexican human sera: Cutting-edge the knowledge.

摘要: Clinical diagnosis of leishmaniasis is highly complex, presenting a wide range clinical manifestations, sometimes non-specific, and thus the epidemiological study diagnostic need specific molecular markers for each Leishmania species. spp. posses different Fe-SOD isoforms, one which excreted into external milieu and, immunogenic characteristics, very reliable marker. Superoxide dismutases (SODs) are antioxidant metal-enzymes responsible dismutation superoxide ion hydrogen peroxide oxygen, it considered an important virulence factor. In this manuscript we have purified iron(Fe)-SOD by braziliensis using ion-exchange molecular-sieve chromatography studied as antigen in serodiagnostic analyses ELISA Western blot techniques, testing 213 human sera from Mexico. Indeed, L. Fe-SODe has been 123.26 times with activity about 893.66 U/mg protein. Applying enzymes serological tests found 17.84% positive. We demonstrated that enzyme more sensitive than non-purified ones also demonstrated, first time, presence antibodies against braziliensis, not main species country, population Hidalgo Nuevo Leon States.