Molecular species identification of Trichuris trichiura in African green monkey on St. Kitts, West Indies.

摘要: The population of African green monkeys (AGM, Chlorocebus aethiops sabaeus) on St. Kitts, West Indies is believed to be as large or greater than the human population. Interactions with humans are frequent and pathogens carried by AGM, such Trichuris spp., may pose a risk humans. objectives this study were assess use molecular methods for diagnosing spp. in AGM compare its DNA sequences those found other non-human primates Fecal samples collected from trapped individually housed between January December 2015 analysed using fecal flotation Sheather's sugar solution PCR amplification sequencing 18S rRNA ITS2 fragments. Phylogenetic analysis was performed. 91% (81/89) 55.4% (31/56) positive PCR, respectively. Both AGM-NADH1 gene T. trichiura-18S showed no variations sequence 100% identical corresponding deposited GenBank. Nevertheless some diversities among 12 sequences, which <5%. put Kittitian into same clades trichiura many geographical regions. These data confirm that reservoirs We suggest one health approach curtail enteric parasitic infections populations insular country.