CARM1 is required in embryonic stem cells to maintain pluripotency and resist differentiation.

摘要: Histone H3 methylation at R17 and R26 recently emerged as a novel epigenetic mechanism regulating pluripotency in mouse embryos. Blastomeres of four-cell embryos with high these sites show unrestricted potential, whereas those lower levels cannot support development when aggregated chimeras like cells. Increasing histone methylation, through expression coactivator-associated-protein-arginine-methyltransferase 1 (CARM1) embryos, elevates key genes directs cells to the pluripotent inner cell mass. We demonstrate CARM1 is also required for self-renewal embryonic stem (ES) In ES cells, depletion downregulates leading their differentiation. associates Oct4/Pou5f1 Sox2 promoters that display detectable R17/26 methylation. overexpressing arginine Nanog promoter which now associates. Such express elevated delay response differentiation signals. Thus, embryo blastomeres, by allows modulation pluripotency.