Formation and activity of phaseollin in the interaction between bean hypocotyls (Phaseolus vulgaris) and physiological races of Colletotrichum lindemuthianum

摘要: Abstract Quantitative measurements, using thin layer chromatography plus ultraviolet spectophotometry and gas-liquid chromatography, have shown that phaseollin accumulation was limited to infected tissue which visibly brown. Phaseollin accumulated much earlier following infection by an incompatible race, caused hypersensitivity, than a compatible race eventually lesions form. Basal metabolism not affected the extensive intracellular growth of races Colletotrichum lindemuthianum continued for several days before host cells collapsed became Concentrations 71 147 μg phaseollin/g fresh weight excised hypersensitive were detected. However, calculations suggest concentrations in might be many-fold higher these values. Germination all equally sensitive low (less 10 μg/ml). Although germ-tube also inhibited small amounts phaseollin, mycelial less experiments indicated metabolized hyphae liquid culture. The production, cellular distribution concentration, fungitoxicity are discussed relation importance restricting hyphal during reactions.