Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture.
作者： Yi-Meng Duan , Ying Jin , Meng-Li Guo , Leng-Xin Duan , Jian-Gang Wang
DOI: 10.7150/JCA.26339
关键词:
摘要: Gecko (Gekko japonicus) extracts have been used in traditional Chinese medicine for many years. It has proven that the gecko polypeptide mixture (GPM) extracted from can inhibit growth of multiple types tumor cells. In order to investigate possible anti-tumor molecular mechanisms GPM, we RNA-seq technology identify differentially expressed genes (DEGs) human hepatocellular carcinoma (HCC) HepG2 cells treated with or without GPM. MTT assay was detect viability DAPI fluorescence staining performed observe morphological changes nuclei Western blot analysis applied expressions apoptosis-related and endoplasmic reticulum stress (ERS)-related proteins Flow cytometry apoptosis reactive oxygen species (ROS) Our results showed GPM inhibited proliferation induced suggested ER-nucleus signaling pathway involved anti-cancer mechanism Therefore, may induce via ERs pathway.
sci-hub.se 参考文章(41)
Wenjin Bu, Tianyou Luo, miR-1297 Promotes Cell Proliferation of Non-Small Cell Lung Cancer Cells: Involving in PTEN/Akt/Skp2 Signaling Pathway. DNA and Cell Biology. ,vol. 36, pp. 976- 982 ,(2017) , 10.1089/DNA.2017.3886
Whasun Lim, Changwon Yang, Muhah Jeong, Fuller W Bazer, Gwonhwa Song, Coumestrol induces mitochondrial dysfunction by stimulating ROS production and calcium ion influx into mitochondria in human placental choriocarcinoma cells. Molecular Human Reproduction. ,vol. 23, pp. 786- 802 ,(2017) , 10.1093/MOLEHR/GAX052
Chun-ling Wang, Chuan Liu, Li-li Niu, Li-rui Wang, Li-hua Hou, Xiao-hong Cao, Surfactin-Induced Apoptosis Through ROS–ERS–Ca2+-ERK Pathways in HepG2 Cells Cell Biochemistry and Biophysics. ,vol. 67, pp. 1433- 1439 ,(2013) , 10.1007/S12013-013-9676-7
Ying Song, Jian-Gang Wang, Rui-Fang Li, Yan Li, Zhao-Chu Cui, Leng-Xin Duan, Fei Lu, Gecko crude peptides induce apoptosis in human liver carcinoma cells in vitro and exert antitumor activity in a mouse ascites H22 xenograft model. BioMed Research International. ,vol. 2012, pp. 743573- 743573 ,(2012) , 10.1155/2012/743573
George Paxinos, Erika Gyengesi, Zane Bruce Andrews, Oxidative Stress in the Hypothalamus: the Importance of Calcium Signaling and Mitochondrial ROS in Body Weight Regulation. Current Neuropharmacology. ,vol. 10, pp. 344- 353 ,(2012) , 10.2174/157015912804143496
Marianna Lucafò, Marco Gerdol, Alberto Pallavicini, Sabrina Pacor, Sonia Zorzet, Tatiana Da Ros, Maurizio Prato, Gianni Sava, Profiling the molecular mechanism of fullerene cytotoxicity on tumor cells by RNA-seq Toxicology. ,vol. 314, pp. 183- 192 ,(2013) , 10.1016/J.TOX.2013.10.001
Liu Yi, Yang Zongyuan, Gong Cheng, Zhang Lingyun, Yu GuiLian, Gong Wei, Quercetin enhances apoptotic effect of tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) in ovarian cancer cells through reactive oxygen species (ROS) mediated CCAAT enhancer‐binding protein homologous protein (CHOP)‐death receptor 5 pathway Cancer Science. ,vol. 105, pp. 520- 527 ,(2014) , 10.1111/CAS.12395
Renata Sano, John C. Reed, ER stress-induced cell death mechanisms Biochimica et Biophysica Acta. ,vol. 1833, pp. 3460- 3470 ,(2013) , 10.1016/J.BBAMCR.2013.06.028
Gökhan S. Hotamisligil, Endoplasmic Reticulum Stress and the Inflammatory Basis of Metabolic Disease Cell. ,vol. 140, pp. 900- 917 ,(2010) , 10.1016/J.CELL.2010.02.034
Fei Liu, Jian-Gang Wang, Shu-Ying Wang, Yan Li, Yin-Ping Wu, Shou-Min Xi, Antitumor effect and mechanism of Gecko on human esophageal carcinoma cell lines in vitro and xenografted sarcoma 180 in Kunming mice. World Journal of Gastroenterology. ,vol. 14, pp. 3990- 3996 ,(2008) , 10.3748/WJG.14.3990