Perturbation of DNA repair gene expression due to interspecies hybridization.

摘要: Abstract The effect of interspecies hybridization on gene regulation was examined using real-time polymerase chain reaction (RT-PCR) to measure the expression five base-excision repair genes in brain, eye, gill, liver, and tailfin tissues from Xiphophorus parental species F1 hybrids. Relative mRNA levels uracil N-glycosylase (Ung), Apurinic/apyrimidinic endonuclease (Ape1), polymerase-β (Polb), flap (Fen1), DNA ligase (Lig1) were measured three (X. maculatus Jp 163 B, X. helleri Sarabia, andersi andC) two hybrids, Sp-helleri hybrid B × X. Sarabia) Sp-andersi andersi) identify that undergo changes upon hybridization. Significant differences observed between animals their respective hybrids both crosses. Generally, marked increases across all cross compared either or parents. In contrast, generally exhibited decreased expression, although this trend more specific individual than for