Novel antimalarial antibodies highlight the importance of the antibody Fc region in mediating protection.
作者： Richard J. Pleass , Solabomi A. Ogun , David H. McGuinness , Jan G. J. van de Winkel , Anthony A. Holder
DOI: 10.1182/BLOOD-2003-02-0583
关键词:
摘要: Parasite drug resistance and difficulties in developing effective vaccines have precipitated the search for alternative therapies malaria. The success of passive immunization suggests that immunoglobulin (Ig)-based are effective. To further explore mechanism(s) by which antibody mediates its protective effect, we generated human chimeric IgG1 IgA1 a single-chain diabody specific C-terminal 19-kDa region Plasmodium yoelii merozoite surface protein 1 (MSP119), major target immune responses. These novel reagents triggered vitro phagocytosis merozoites but, unlike their parental mouse IgG2b, failed to protect against parasite challenge vivo. Therefore, Fc appears critical mediating protection vivo, at least this MSP119 epitope. Such antibodies may serve as prototype therapeutic agents, useful tools development neutralization assays with parasites. (Blood. 2003;102:4424-4430)
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