A method for the selection of deletion mutations in the L-proline catabolism gene cluster of Aspergillus nidulans.

摘要: Interest in the selection of mutations affecting L-proline catabolism Aspergillus nidulans is heightened by involvement one very few examples a cluster functionally related genes an eukaryote and increasing awareness biological phenomena which proline participate. The sasA-60 (semialdehyde sensitive) mutation A. results toxicity catabolic precursors L-glutamic γ-semialdehyde (or its internal Schiff base L-Δ1-pyrroline-5-carboxylate) succinic semialdehyde, apparently without pathways concerned. As unlinked to prn gene cluster, specifying products necessary for as L-proline, precursor γ-semialdehyde, highly toxic strains, this forms basis powerful positive technique obtaining number types mutations. Many these can be directly classified according product(s) affected on growth phenotype with respect L-arginine L-ornithine utilization, proline-dependent resistance certain amino acid analogues effect supplementation auxotrophies. availability both extensive nutritional screening system has enabled identification fourteen spontaneous deletion mutations, recognized extending into prnB gene, principal permease, at least other gene. These have been partially characterized genetically biochemically. In particular their use greatly facilitated fine-structure mapping aided studies regulation expression.