Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor

摘要: Abstract The differential response of cultured parsley cells to u.v. irradiation and elicitor treatment is a paradigm for analysis specific plant defense responses. We demonstrate that freshly isolated protoplasts, in the absence detectable cell wall, maintain fully ability be activated by these important environmental factors. Stimulated protoplasts synthesize typical qualitative patterns amounts potentially protective flavonoid glycosides coumarin phytoalexins following either or with fungal elicitor, respectively. Induced accumulation mRNAs enzymes phenylpropanoid biosynthetic pathways nearly identical cells. Stimulation requires only short period contact, which not sufficient wall regeneration. Importantly, there no activation during protoplast preparation. These results establish respond appropriately two physically distinct stimuli might serve as an especially suitable system signal transduction gene activation.