Flavonoid inhibition of human basophil histamine release stimulated by various agents.

摘要: Eleven naturally occurring flavonoids representing five different chemical classes were studied for their effects on human basophil histamine release triggered by six stimuli. The included flavone, quercetin, taxifolin, chalcone, apigenin, fisetin, rutin, phloretin, tangeretin, hesperetin, and naringin. stimuli antigen, anti-IgE, concanavalin A, ionophore A23187, formylmethionylleucylphenylalanine, tetradecanoyl phorbol acetate. Concentration-effect relationships established each flavonoid (5-50 microM) at concentrations of which produced near optimal release. Variable degrees inhibition noted depending the nature stimulus structure. flavonols, quercetin exhibited a predilection to inhibit stimulated IgE-dependent ligands (antigen, con A). flavanone derivatives, taxifolin inactive, as glycosides, rutin open chain congeners, chalcone also possessed inhibitory activity. Thus, may be useful probes in comparative analysis secretory phenomena. findings suggest that biochemical pathways leading secretion differ subtly from one another. differences are detectable with structures possibly reflect distinct Ca2+ mobilization or other unique mechanisms action.