The synaptophysin/synaptobrevin interaction critically depends on the cholesterol content
作者： Diana Mitter , Clemens Reisinger , Britta Hinz , Susanne Hollmann , Sowmya V. Yelamanchili
DOI: 10.1046/J.1471-4159.2003.01258.X
关键词:
摘要: Synaptophysin interacts with synaptobrevin in membranes of adult small synaptic vesicles. The synaptophysin/synaptobrevin complex promotes to built up functional SNARE complexes thereby modulating efficiency. addition is a cholesterol-binding protein. Depleting the membranous cholesterol content by filipin or beta-methylcyclodextrin (beta-MCD) decreased solubility synaptophysin Triton X-100 less effects on synaptobrevin. In vesicles from rat brain was diminished upon beta-MCD treatment as revealed chemical cross-linking. Mice genetic mutation Niemann-Pick C1 gene developing defect sorting showed significantly reduced amounts compared their homo- heterozygous littermates. Finally when using primary cultures mouse hippocampus down-regulated after depleting endogenous HMG-CoA-reductase inhibitor lovastatin. Alternatively, up-regulated interaction these cultures. These data indicate that critically depends high membrane Variations availability may promote impair efficiency interfering this complex.
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