Positive Control of Endolysin Synthesis In Vitro by the Gene N Protein of Phage λ

摘要: Abstract Positive control in vitro by gene N protein of bacteriophage λ was demonstrated. DNA used to direct synthesis endolysin a cell-free protein-synthesizing preparation derived from Escherichia coli. The depends on the concomitant protein. When N- preparation, made only if extract added cells which prophage had been induced. use various deletion strains proved that this stimulating activity vivo is coded known gene, it must be N. ability stimulate template, therefore, constitutes an assay for