Low potential biofuel cell anodes based on redox polymers with covalently bound phenothiazine derivatives for wiring flavin adenine dinucleotide-dependent enzymes

摘要: Abstract The design of biofuel cell anodes with substantially decreased potential is a prerequisite for the development cells large open-circuit voltage and power density. Redox polymers covalently attached phenothiazine derivatives such thionine acetate, toluidine blue, azure B simultaneously providing epoxide functions covalent binding to suitably modified electrode surfaces crosslinking were synthesized evaluated their ability transfer electrons from FAD cofactor flavodehydrogenase domain cellobiose dehydrogenase Myriococcum thermophilum (FAD- Mt CDH), Corynascus thermophilus Ct or glucose oxidase Aspergillus niger (GOx). Polymer/enzyme films bound via polymer epoxy groups terminal amino introduced graphite by electrochemically induced grafting diaminoheptane Boc-protected ethylene diamine (EDA). electrodes optimized biocatalytic oxidation respect hydrophilicity backbone, nature derivative, pH value, as well relative amount enzyme, crosslinker. Biofuel based on blue-modified redox integrated FAD- CDH, GOx in combination bilirubin biocathode exhibited voltages more than 0.7 V maximum densities range 4 6 μW cm −2 at value 7.8.