Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆TK/∆gE.

摘要: The outbreak of pseudorabies in China, caused by more virulent virus (PRV) than the classical strains, has led to considerable economic losses. In this study, PRV strain HNXY was isolated from Henan province China 2015 pig farm with severe reproductive failure sows and a high mortality piglets. 50% tissue culture infectious doses (TCID50) Vero cells were examined be 106.5/mL, neutralisation titer against Bartha-K61 significantly higher when tested serum vaccinated pigs. lethal (LD50) six-week-old BALB/c mice two-month-old PRV-free pigs both 102.3 TCID50. classified as genotype II, numerous amino acid variations found gB, gE, gC, gD, TK, RR1 proteins, compared other countries or those prevalent before 2012. attenuated rHNXY-∆TK/∆gE further constructed, which presented smaller plaques HNXY, well similar growth kinetics. confirmed non-pathogenic zero-day-old This study updated promising develop into new vaccine candidate.