Experimental challenge with bovine respiratory syncytial virus in dairy calves: bronchial lymph node transcriptome response

摘要: Bovine Respiratory Disease (BRD) is the leading cause of mortality in calves. The objective this study was to examine response host’s bronchial lymph node transcriptome Syncytial Virus (BRSV) a controlled viral challenge. Holstein-Friesian calves were either inoculated with virus (103.5 TCID50/ml × 15 ml) (n = 12) or mock challenged phosphate buffered saline (n = 6). Clinical signs scored daily and blood collected for haematology counts, until euthanasia at day 7 post-challenge. RNA extracted sequenced (75 bp paired-end) from nodes. Sequence reads aligned UMD3.1 bovine reference genome differential gene expression analysis performed using EdgeR. There clear separation between BRSV control based on changes, despite an observed mild clinical manifestation disease. Therefore, measuring host levels may be beneficial diagnosis subclinical BRD. 934 differentially expressed genes (DEG) (p  2) Over-represented ontology terms, pathways molecular functions, among DEG, associated immune responses. top enriched included interferon signaling, granzyme B signaling pathogen pattern recognition receptors, which are responsible cytotoxic responses necessary eliminate virus.