In vitro effects of 2-hydroxyestradiol-17β on ovarian follicular steroid secretion in the catfish Heteropneustes fossilis and identification of the receptor and signaling mechanisms
作者： T.K. Chourasia , K.P. Joy
DOI: 10.1016/J.YGCEN.2011.12.007
关键词:
摘要: Abstract Ovarian pieces containing postvitellogenic follicles were incubated in vitro with different concentrations of the catecholestrogen 2-hydroxyestradiol-17β (2-OHE 2 ) to evaluate its effects on steroid production and germinal vesicle breakdown (GVBD) catfish Heteropneustes fossilis . The incubation 2-OHE induced a shift steroidogenic pattern: C 19 18 steroids testosterone (T) estradiol-17β (E ), respectively significantly decreased concomitant significant increase 21 progesterone (P 4 17-hydroxyprogesterone (17-OHP), 17,20β-dihydroxy-4-pregnen-3-one (17,20β-DP), 17,20α-dihydroxy-4-pregnen-3-one (17,20α-DP) cortisol (F). Concomitantly, dose-dependently GVBD response, first sign meiosis resumption. co- pre-incubations ovarian , adrenergic (phentolamine, α-blocker propranolol, β-blocker) or estrogen (tamoxifen) receptor blockers resulted inhibition stimulatory effect reversed E was more effective than β-blocker. Our results suggest that appears employ both (α-type) mechanisms mediating effects. pre-incubation IBMX (a cAMP elevating drug), H89 protein kinase A inhibitor), PD098059 MAP inhibitor) inhibited steroids. chelerythrine other hand, varied condition. In co-incubation, showed effects: 17,20β-DP, elevated, P 17-OHP decreased. set up, all except by higher groups. Taken together, data involvement cAMP–protein A, pathways modulation activity.
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Hanna Rosenfeld, Iris Meiri, Abigail Elizur, Gonadotropic regulation of oocyte development The Fish Oocyte. pp. 175- 202 ,(2007) , 10.1007/978-1-4020-6235-3_7
Yoshitaka Nagahama, Gonadotropin action on gametogenesis and steroidogenesis in teleost gonads Zoological Science. ,vol. 4, pp. 209- 222 ,(1987)
Frederick W. Goetz, 3 Hormonal Control of Oocyte Final Maturation and Ovulation in Fishes Fish Physiology. ,vol. 9, pp. 117- 170 ,(1983) , 10.1016/S1546-5098(08)60303-9
Yoshitaka Nagahama, Michiyasu Yoshikuni, Masakane Yamashita, Minoru Tanaka, 13 Regulation of Oocyte Maturation in Fish Fish Physiology. ,vol. 13, pp. 393- 439 ,(1994) , 10.1016/S1546-5098(08)60074-6
Yoshitaka Nagahama, Masakane Yamashita, Regulation of oocyte maturation in fish. Development Growth & Differentiation. ,vol. 50, pp. 393- 439 ,(2008) , 10.1111/J.1440-169X.2008.01019.X
Edward M. Donaldson, George A. Hunter, 7 Induced Final Maturation, Ovulation, and Spermiation in Cultured Fish Fish Physiology. ,vol. 9, pp. 351- 403 ,(1983) , 10.1016/S1546-5098(08)60307-6
T.K. Chourasia, K.P. Joy, Seasonal variation in tissue estrogen-2/4-hydroxylases (EH) and in vitro effects of steroids on ovarian EH activity in the catfish Heteropneustes fossilis. Steroids. ,vol. 75, pp. 1097- 1105 ,(2010) , 10.1016/J.STEROIDS.2010.07.004
R. van den Hurk, W.G.E.J. Schoonen, G.A. van Zoelen, J.G.D. Lambert, The biosynthesis of steroid glucuronides in the testis of the zebrafish, Brachydanio rerio, and their pheromonal function as ovulation inducers. General and Comparative Endocrinology. ,vol. 68, pp. 179- 188 ,(1987) , 10.1016/0016-6480(87)90027-X
Leon J. Spicer, James M. Hammond, Regulation of ovarian function by catecholestrogens: Current concepts Journal of Steroid Biochemistry. ,vol. 33, pp. 489- 501 ,(1989) , 10.1016/0022-4731(89)90033-2
S. K. Das, J. A. Taylor, K. S. Korach, B. C. Paria, S. K. Dey, D. B. Lubahn, Estrogenic responses in estrogen receptor-α deficient mice reveal a distinct estrogen signaling pathway Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 12786- 12791 ,(1997) , 10.1073/PNAS.94.24.12786