Immobilization-free programmable hairpin probe for ultrasensitive electronic monitoring of nucleic acid based on a biphasic reaction mode.

摘要: This work designs a novel programmable hairpin probe (PHP) for the immobilization-free electrochemical detection of nucleic acid by coupling polymerase/nicking-induced isothermal signal amplification strategy with biphasic reaction mode first time. The designed PHP (including target-recognition region, template sequence enzymatic and an inactivated anti-streptavidin aptamer) could program multiple reactions in solution phase accompanying situ amplified detectable at electrode surface labeled ferrocene tag on PHP. Upon addition target analyte into solution, DNA initially hybridized recognition region Replication-induced strand-displacement generated activated aptamer assistance polymerase. Then, polymerase/nicking enzymes cleave polymerize repeatedly replication product, thus resulting formation numerous template-complementary initi...