Profiling of differentially expressed genes in human stem cells by cDNA microarray.

摘要: Stem cells are unique cell populations with the ability to undergo both self-renewal and differentiation, although a wide variety of adult stem as well embryonic have been identified plasticity has recently reported. To identify genes implicated in control state characteristics each line, we analyzed expression profiles human embryonic, hematopoietic (CD34+ CD133+), mesenchymal using cDNA microarrays, that were differentially expressed specific populations. In particular able potential hESC signature-like encode transcription factors (TFAP2C MYCN), an RNA binding protein (IMP-3), functionally uncharacterized (MAGEA4). The overlapping sets 22 up-regulated 141 down-regulated this study three types may also provide insight into developmental mechanisms common all cells. Furthermore, our comprehensive analyses gene various help genetic pathways involved multi-lineage differentiation.