Functional Analysis of a Cell Cycle–Associated, Tumor-Suppressive Gene, Protein Tyrosine Phosphatase Receptor Type G, in Nasopharyngeal Carcinoma

摘要: Functional studies to identify the potential role of a chromosome 3p14-21 gene, protein tyrosine phosphatase receptor type G (PTPRG), were performed. PTPRG was identified as candidate tumor suppressor gene (TSG) in nasopharyngeal carcinoma (NPC) by differential profiling tumorigenic and nontumorigenic NPC 3 microcell hybrids (MCH). Down-regulation this found segregants when compared with their corresponding tumor-suppressive MCHs, well cell lines biopsies. Promoter hypermethylation loss heterozygosity be important mechanisms contributing silencing. overexpression induces growth suppression reduced anchorage-independent vitro. This is first study use tetracycline-responsive vector expression system stable transfectants. Results indicate its ability induce significant nude mice under conditions activating transgene expression. These now provide functional evidence indicating critical interactions extracellular matrix milieu arrest changes cycle status. associated inhibition pRB phosphorylation through down-regulation cyclin D1. novel findings enhance our current understanding how may contribute tumorigenesis.