Hit and Run Transcriptional Repressors Are Difficult to Catch in the Act.
作者： Manan Shah , Alister P. W. Funnell , Kate G. R. Quinlan , Merlin Crossley
关键词:
摘要: Transcriptional silencing may not necessarily depend on the continuous residence of a sequence-specific repressor at control element and act via "hit run" mechanism. Due to limitations in assays that detect transcription factor (TF) binding, such as chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), this phenomenon be challenging therefore its prevalence underappreciated. To explore possibility, erythroid gene promoters are regulated directly GATA1 an inducible system analyzed. It is found many genes bound immediately after induction but residency decreases over time, particularly repressed genes. Furthermore, it shown repressive mark H3K27me3 seldom associated with repressors, whereas, contrast, active (H3K4me3) histone overwhelmingly TF binding. hypothesized during cellular differentiation development, certain silenced TFs subsequently vacate region. Catching ChIP-seq thus temporally prospect. The use systems, epitope tags, alternative techniques provide opportunities for detecting elusive transcriptional silencing. Also see video abstract here https://youtu.be/vgrsoP_HF3g.
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