Immunochemical evidence for the involvement of prostaglandin H synthase in hydroperoxide-dependent oxidations by ram seminal vesicle microsomes.
作者： W R Pagels , R J Sachs , L J Marnett , D L Dewitt , J S Day
DOI: 10.1016/S0021-9258(18)32442-6
关键词:
摘要: Monoclonal antibodies against prostaglandin H (PGH) synthase have been used to precipitate cyclooxygenase and peroxidase activities from detergent-solubilized preparations of ram seminal vesicle microsomes. Approximately 85% the solubilized activity was precipitated using an excess anti-PGH antibody; under similar conditions, immunoprecipitation 60% diphenylisobenzofuran peroxidase, 75% phenylbutazone 50% epinephrine occurred. In contrast, less than 10% or could be with nonimmune, control antibody preparations. These data indicate that hydroperoxidase PGH is major catalyzing co-oxidation xenobiotics in
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