Development and Screening of Subtractive RNAi Libraries from Ovarian Cancer Cell Lines

摘要: Abstract : The Specific aims of this proposal are: (1) Construction two subtractive RNAi libraries targeting mRNA transcripts predominantly present in an (a) advanced ovarian cancer cell line and (b) metastatic line. (2) Screen for genes vitally important survival growth. malignant lines will be transfected with individual clones from the effect on viability monitored. Clones producing marked growth inhibition or causing apoptosis sequenced targeted identified by BLAST search. (3) Validation targets: a) confirmation inhibitory/apoptotic selected clones; b) verification translation respective mRNAs Western blot; c) increased transcription target RT-PCR blot. proposed studies are expected to first, but very important, step towards identification targets therapy cancer. Later scope research widened including samples patients confirm data eventually, obtain additional information. great advantage our approach, compared other modern methods (such as microarrays total transcriptome libraries)is relative simplicity much lower costs.