Immature reticulocyte fraction reference control and related methods

摘要: A composition (and associated methods) including a plurality of treated red blood cells for simulating reticulocytes, and particularly an immature reticulocyte fraction, whole when processed as sample in automated analyzer capable detecting reticulocytes. method making the or other simulated reticuolcyte may include steps contacting suspension each having membrane initial state that surrounds interior volume cell with effective amount hypertonic permeabilizing solution dimethyl sulfoxide hypotonic loading agent delivery agent, sufficient time to form pores membrane, permitting enter into cells, and, after entry desired cell, sealing substantially restoring while encapsulating within resulting preferably also retaining substantial hemoglobin from original starting cell.