The impact of biochemical methods for single muscle fibre analysis.

摘要: Biochemical methods for single muscle fibre analysis provide sensitive measures elucidating heterogeneity. The understanding of the complexity skeletal fibres, initially based on qualitative histochemistry and immunohistochemistry, has been greatly expanded by quantitative micromethods, such as microphotometry microbiochemical assays. Assessment metabolic enzyme activity levels revealed pronounced scattering within between different types highlighted use specific ratios discriminative measures. With exception type I properties are loosely coupled with molecular myofibrillar apparatus. As such, myosin heavy chain (MHC) isoforms appear to be best choice delineation. Among two available MHC-based distinction, electrophoresis appears superior immunohistochemistry. electrophoretic separation MHC in fibres is and, opposed yields important information isoform proportions hybrid fibres. Histochemical staining ATPase can, thus, correlated most cases profiles. Single studies have demonstrated a relationship ATP phosphorylation potential complement. This corresponds tension costs provides an additional rationale diversity transitions. combination reverse transcriptase (RT) polymerase reaction (PCR) proved highly tool extended level mRNA isoforms. Application RT-PCR techniques fragments identified their protein profile, insights at expression our knowledge plasticity dynamical state