Determination of Microcirculatory Changes and Angiogenesis in a Model of Frostbite Injury In Vivo

摘要: Background. The breakdown of skin microcirculation and the leukocyte–endothelium interaction are assumed to play a key role in pathophysiology frostbite injuries. However, little is known as yet. aim was develop an vivo model monitor microcirculatory changes angiogenesis after injury. Materials Methods. Deep partial thickness injuries were inflicted with no-touchtechnique ears hairless mice (n[ 9). To this end, gas jet nitrogen vapor (T [ –195,8 ± 2.7 � C) delivered onto area 1.9 mm 2 for 1,5 s. Intravital fluorescent microscopy combination FITCdextran Rhodamin 6 G dyes used assess changes, leukocyte behavior, during 14 d wound healing. Results. no perfusion decreased significantly over observed period, almost completely restored due by (day 1: 1.89 [mm ] 0.44SEM, 14: 0.02 0.01). No post-traumatic extension trauma could be observed. Edema formation increased up 7. number adherent leukocytes showed significant increase first 7 d. Functional vessel density post-frostbite decrease 60% baseline value. Conclusions. This novel provides simple nonetheless highly effective technique creating locally limited reproducible using touch technique. Tissue damage can fully attributed thermal trauma, allows repetitive intravital microcirculation, leukocyte– endothelium interaction, angiogenesis. 2011