Stimulation of goblet cell mucous secretion by activation of nerves in rat conjunctiva

摘要: An epithelial debridement wound, as a stimulus to the cornea, causes conjunctival goblet cell mucous secretion in that eye. To determine if this stimulation of is neurally mediated, rats were anesthetized and local anesthetic lidocaine (1%) or buffer alone was administered topically and/or subconjunctivally for 15 min. A corneal wound made one The contralateral eye served control. After 5-120 min, animals sacrificed inferior bulbar buttons removed. Mucus cells stained with Alcian blue periodic acid-Schiff's reagent indicate mucin-containing cells. number cells/0.16 mm2 determined by light microscopy; decrease indicated an increase secretion. Stimulation wounding induced Secretion observed rapidly 5 min after long 120 Topical application lidocaine, subconjunctival injection combination both inhibited wound-induced We conclude can be mediated could serve immediate response protect ocular surface.