Chromatin accessibility dynamics of Chlamydia -infected epithelial cells

摘要: Chlamydia are Gram-negative, obligate intracellular bacterial pathogens responsible for a broad spectrum of human and animal diseases. In humans, trachomatis is the most prevalent sexually transmitted infection worldwide causative agent trachoma (infectious blindness) in disadvantaged populations. Over course its developmental cycle, extensively remodels niche parasitises host cell nutrients, with substantial resulting changes to transcriptome proteome. However, little information available on impact chlamydial epigenome global gene regulation. Regions open eukaryotic chromatin correspond nucleosome-depleted regions, which turn associated regulatory functions transcription factor binding. We applied formaldehyde-assisted isolation elements enrichment followed by sequencing (FAIRE-Seq) generate temporal maps C. trachomatis-infected epithelial cells vitro over cycle. detected both conserved distinct genome-wide accessibility infection. The observed differentially accessible regions include temporally-enriched sets factors, may help shape response These motifs were linked genomic features genes immune responses, re-direction signalling, cell-cell adhesion, extracellular matrix, metabolism apoptosis. This work provides another perspective complex infection, will inform further studies transcriptional regulation Chlamydia-infected tissues.