摘要: A detailed understanding of the mechanism chromosome replication in eukaryotic cell and fidelity this process requires a comprehensive biochemical genetic characterization DNA polymerases their accessory factors. Genetic manipulation yeast Saccharomyces cerevisiae has shown that at least three nuclear are essential for viability. These products POL1 (also designated CDC17), POL2, POL3 (CDC2) genes (Johnson, et al, 1985, Morrison al., 1990, Sitney 1989, Boulet 1989). Extensive structural functional similarity between mammalian cells many components apparatus including replicative prompted us to propose unified nomenclature (Burgers 1990). The catalytic subunit polymerase a, previously called I yeast, is encoded by gene (Johnson 1985). Polα 4-subunit enzyme contains an associated primase activity. One still unsolved problem biochemistry whether possesses proofreading exonuclease activity (reviewed Burgers, Whereas does not have detectable 3’-5’-exonuclease activity, purified monomelic polypeptide exhibited such one, but another study (Kunkel Brooke 1991). Polδ, PolIII product (Sitney 2-subunit 3′-5′-exonuclease capable vitro (Bauer, G.A. 1988). Pole, PolII, POL2 gene.
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