Alternative oxidase: what information can protein sequence comparisons give us?

摘要: The finding that alternative oxidase (AOX) is present in most kingdoms of life has resulted a large number AOX sequences are available for analyses. Multiple sequence alignments proteins from evolutionarily divergent organisms represent valuable tool and can be used to identify amino acids domains may play role catalysis, membrane association post-translational regulation, especially when these data coupled with the structural model enzyme. I validate use this approach by demonstrating it detects conserved glutamate histidine residues initially led its identification as di-iron carboxylate protein generation protein. A comparative analysis using larger dataset identified 35 additional all AOXs examined, 30 which have not been investigated date. hypothesize will involved quinol terminal activity or AOX. Major differences between revealed, hypothesized two angiosperm-specific responsible non-covalent dimerization AOX, whereas indels aplastidic their regulation. scheme predicting whether particular recognized monoclonal antibody generated against Sauromatum guttatum (Voodoo lily) presented. functional sites greater than expected, determining structure prove extremely future research.