The Flavoenzyme Ferredoxin (Flavodoxin)-NADP(H) Reductase Modulates NADP(H) Homeostasis during the soxRS Response of Escherichia coli

作者: Adriana R Krapp , Ramiro E Rodriguez , Hugo O Poli , Darío H Paladini , Javier F Palatnik

DOI: 10.1128/JB.184.5.1474-1480.2002

关键词:

摘要: Escherichia coli cells from strain fpr, deficient in the soxRS-induced ferredoxin (flavodoxin)-NADP(H) reductase (FPR), display abnormal sensitivity to bactericidal effects of superoxide-generating reagent methyl viologen (MV). Neither bacteriostatic nor inactivation oxidant-sensitive hydrolyases could be detected fpr exposed MV. FPR did not affect MV-driven soxRS response, whereas overexpression led enhanced stimulation regulon, with concomitant oxidation NADPH pool. Accumulation a site-directed mutant that uses NAD(H) instead NADP(H) had no effect on induction and failed protect MV toxicity, suggesting contributes homeostasis stressed bacteria.

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