Layer-by-Layer Assembly of DNA- and Protein-Containing Films on Microneedles for Drug Delivery to the Skin

摘要: Microneedle patches contain micrometer-scale needles coated with bioactive agents for minimally invasive drug delivery to the skin. In this study, we introduce layer-by-layer approaches fabrication of ultrathin DNA- and protein-containing polyelectrolyte films (or "polyelectrolyte multilayers", PEMs) on surfaces stainless steel microneedles. DNA-containing PEMs were fabricated microneedles by alternating deposition plasmid DNA a hydrolytically degradable poly(β-amino ester). Protein-containing using sodium poly(styrene sulfonate) (SPS) bovine pancreatic ribonuclease A (RNase A) conjugated synthetic protein transduction domain. Layer-by-layer assembly resulted in ultrathin, uniform, defect-free coatings microneedles, as characterized fluorescence microscopy. These eroded thereby released or when incubated saline inserted into porcine cadaver skin deposited along edges microneedle tracks depths ∼500 600 μm. We conclude that PEM-coated offer novel useful approach transdermal protein-based therapeutics could also prove other applications.