Enzymic synthesis of polynucleotides. I. Polynucleotide phosphorylase of azotobacter vinelandii.
作者： Marianne Grunberg-Manago , Priscilla J. Ortiz , Severo Ochoa
DOI: 10.1016/0006-3002(56)90286-4
关键词:
摘要: The isolation, partial purification and some properties of polynucleotide phosphorylase Azotobacter vinelandii are described. enzyme catalyzes the synthesis highly polymerized ribonucleic acid-like polynucleotides from 5′-nucleoside diphosphates with release orthophosphate. reaction requires magnesium ions is reversible. Thus, also phosphorolysis to yield corresponding diphosphates. preparation isolation a number containing one or several kinds mononucleotide units scope, mechanism significance discussed.
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