Mass cultivation of Tetrahymena thermophila yielding high cell densities and short generation times
作者： Thomas Kiy , Arno Tiedtke
DOI: 10.1007/BF00240728
关键词:
摘要: A cheap medium, composed of skimmed milk powder, yeast extract, and glucose, for mass cultivation the protozoon Tetrahymena thermophila has been developed. Cell concentrations 5 x 106 cells/ml unprecedented short generation times only 1.4 h were determined in batch cultures. During exponential phase growth, daughter cells initiated a new cell division even before previous had completed, resulting formation chains. Addition glucose extended stationary phase. Using bench-top fermentor supplied with digital control unit utilization nutrient components culture was monitored. Milk protein consumed completely, but lactose partly.
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sci-hub.se 参考文章(15)
Donald Lynch Hill, The Biochemistry and Physiology of Tetrahymena ,(2012)
E. Orias, L. Rasmussen, Dual capacity for nutrient uptake in Tetrahymena: IV. Growth without food vacuoles and its implications Experimental Cell Research. ,vol. 102, pp. 127- 137 ,(1976) , 10.1016/0014-4827(76)90307-4
Geoffrey H. Bourne, The biochemistry and physiology of bone Academic Press. ,(1971)
Arno Tiedtke, Purification and properties of secreted N-acetyl-β--hexosaminidase of Tetrahymena thermophila Comparative Biochemistry and Physiology B. ,vol. 75, pp. 239- 243 ,(1983) , 10.1016/0305-0491(83)90321-8
ARTHUR J. REPAK, An Inexpensive and Practical Medium for Rearing Mass-cultures of Tetrahymena Journal of Eukaryotic Microbiology. ,vol. 19, pp. 417- 421 ,(1972) , 10.1111/J.1550-7408.1972.TB03496.X
L. Rasmussen, E. Orias, Dual capacity for nutrient uptake in Tetrahymena. V. Utilization of amino acids and proteins. Journal of Cell Science. ,vol. 36, pp. 343- 353 ,(1979)
ULRICH SCHÖNEFELD, A. WILHELM ALFERMANN, JOACHIM E. SCHULTZ, Economic Mass Cultivation of Paramecium tetraurelia on a 200-Liter Scale† Journal of Eukaryotic Microbiology. ,vol. 33, pp. 222- 225 ,(1986) , 10.1111/J.1550-7408.1986.TB05595.X
L. Rasmussen, Tove Agnete Kludt, Particulate material as a prerequisite for rapid cell multiplication in Tetrahymena cultures Experimental Cell Research. ,vol. 59, pp. 457- 463 ,(1970) , 10.1016/0014-4827(70)90654-3
Wayne G. Landis, Donna M. Haley, Mark V. Haley, Dennis W. Johnson, H. Dupont Durst, Russell E. Savage, Discovery of multiple organofluorophosphate hydrolyzing activities in the protozoan Tetrahymena thermophila. Journal of Applied Toxicology. ,vol. 7, pp. 35- 41 ,(1987) , 10.1002/JAT.2550070107
Jacek S. Ropenga, Maryse Lenfant, Bioconversion of isosorbide dinitrate into isosorbide mononitrate by the protozoan Tetrahymena thermophila: relationship to glutathione transferase levels Applied Microbiology and Biotechnology. ,vol. 26, pp. 117- 119 ,(1987) , 10.1007/BF00253893