Reconstitution of Purple Membrane Vesicles Catalyzing Light-driven Proton Uptake and Adenosine Triphosphate Formation
作者： Efraim Racker , Walther Stoeckenius
DOI: 10.1016/S0021-9258(19)43080-9
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摘要: Abstract The purple membrane from Halobacterium halobium was incorporated into phospholipid vesicles. On illumination (2 x 106 ergs per cm2 s) the reconstituted vesicles took up protons (50 to 200 ng of ions H+ mg protein) which were released in dark. Addition valinomycin accelerated both rate uptake light and release Uncouplers oxidative phosphorylation abolished protons. Inclusion mitochondrial oligomycin-sensitive ATPase during reconstitution yielded catalyzed light-dependent phosphorylation. These represent a simple model system for biological proton pump capable generating ATP ADP Pi.
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