Studies of lectins XXXVI. Properties of some lectins prepared by affinity chromatography on O-glycosyl polyacrylamide gels

摘要: A number of lectins has been purified by affinity chromatography on O-glycosyl polyacrylamide gels. The isolated (and the particular sugar ligands used in carriers) are as follows: Anguilla anguilla, serum (alpha-L-fucosyl-), Vicia cracca, seeds; Phaseolus lunatus, Glycine soja, Dolichos biflorus, Maclura pomifera, Sarothamnus scoparius, Helix pomatia, ablumin glands; Clitocybe nebularis, fruiting bodies (all N-acetyl-alpha-D-galactosaminyl-); Ricinus communis, seeds (beta-lactosyl-); Ononis spinosa, root; Fomes fomentarius, bodies; Marasmius oreades, alpha-D-galactosyl-), Canavalia ensiformis, seeds, (i.e., concanavalin A) (alpha-D-glucosyl-). Physicochemical properties Pomatia and communis corresponded well to preparations studied earlier other workers. For essential physiochemical data (sedimentation coefficient, molecular weight, subunit composition, electrophoretic patterns, amino acid carbohydrate content, isoelectric point) were established their precipitating, hemagglutinating mitogenic activities determined.