Accelerated start-up, long-term performance and microbial community shifts within a novel upflow porous-plated anaerobic reactor treating nitrogen-rich wastewater via ANAMMOX process

摘要: The anaerobic ammonium oxidation (anammox) process has gained much popularity in recent years following its success nitrogen removal. However, not been reported on techniques to promote anammox bacteria immobilization and associated microbial community evolution. In this study, a novel upflow porous-plate reactor (UPPAR) was developed explored biomass retention growth. To comprehend the performance of UPPAR, removal efficiencies, as well dynamics involved process, evaluated reported. When NLR ranging 0.98–1.08 kg m−3 d−1 introduced at various stages UPPAR operation, rapid start-up achieved 63 d, overall rate could reach 90–95%. By end period, it revealed that Proteobacteria abundance had reduced by 43.92% opposed Planctomycetes which increased from 2.95% 43.52%. Conversely, after operated for 124 thus steady-state, most pronounced phylum observed (43.52%) followed (26.63%), Chloroflexi (5.87%), Ignavibacteriae (5.55%), Bacteroidetes (4.9%). Predominant genera included Candidatus Kuenenia – (25.46%) Brocadia (3.15%), an indication mechanism within mainly conducted via autotrophic process. Scanning electron microscopy (SEM) sludge samples obtained steady-state were predominantly granular form with sizes between 2 mm 5 mm. Granules surfaces dominated normal coccoid-shaped cells SEM.