Reactive oxygen species generation by seminal cells during cryopreservation.

摘要: Abstract Objectives. To test the hypothesis that conventionally used procedures for semen cryopreservation may cause an increase in production of reactive oxygen species (ROS) by sperm or seminal leukocytes, which contribute to poor function following cryopreservation. Methods. Eighteen specimens with normal parameters from healthy male donors 22 40 years age were each divided into two portions. The first portion was combined 1:1 Test Yolk Buffer-Glycerol Freezing Medium and frozen gradual cooling liquid nitrogen (−196°C). second washed cells resuspended Sperm Washing (SWM) incubated at room temperature serve as controls. After a period treatment, samples thawed SWM. ROS generation treatment group measured on luminometer. motility, viability, membrane integrity also both control freeze-thaw samples. further assess during process, aliquots purified polymorphonuclear leukocytes (PMNs) separately different conditions (37°C, 22°C, 4°C, −20°C). activity compared other. Results. In PMNs, increased significantly process. highest levels recorded groups when cooled 4°C. extremely low −20°C samples, probably due marked loss cell viability. Conclusions. Gradual reduction process can significant cells. is particularly elevated if sample contaminated more than 0.5 × 10 6 leukocytes. Removal antioxidants prior improve viability function.