作者: Tim Tolker‐Nielsen , Claus Sternberg
DOI: 10.1002/9780471729259.MC01B02S21
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摘要: This unit describes the setup of flow chamber systems for study microbial biofilms, and methods analysis structural biofilm formation. Use chambers allows direct microscopic investigation The biofilms in develop under hydrodynamic conditions, environment can be carefully controlled easily changed. protocols this include construction bubble trap, assembly sterilization system, inoculation chambers, running image capture analysis, disassembly cleaning system. In addition, embedding fluorescent situ hybridization chamber-grown are addressed.