Growing and analyzing biofilms in flow chambers

作者: Tim Tolker‐Nielsen , Claus Sternberg

DOI: 10.1002/9780471729259.MC01B02S21

关键词:

摘要: This unit describes the setup of flow chamber systems for study microbial biofilms, and methods analysis structural biofilm formation. Use chambers allows direct microscopic investigation The biofilms in develop under hydrodynamic conditions, environment can be carefully controlled easily changed. protocols this include construction bubble trap, assembly sterilization system, inoculation chambers, running image capture analysis, disassembly cleaning system. In addition, embedding fluorescent situ hybridization chamber-grown are addressed.

参考文章(17)
G. M. Wolfaardt, J. R. Lawrence, R. D. Robarts, S. J. Caldwell, D. E. Caldwell, Multicellular organization in a degradative biofilm community Applied and Environmental Microbiology. ,vol. 60, pp. 434- 446 ,(1994) , 10.1128/AEM.60.2.434-446.1994
Martin Kuehn, Martina Hausner, Hans-Joachim Bungartz, Michael Wagner, Peter A. Wilderer, Stefan Wuertz, Automated Confocal Laser Scanning Microscopy and Semiautomated Image Processing for Analysis of Biofilms Applied and Environmental Microbiology. ,vol. 64, pp. 4115- 4127 ,(1998) , 10.1128/AEM.64.11.4115-4127.1998
J R Lawrence, D R Korber, B D Hoyle, J W Costerton, D E Caldwell, Optical sectioning of microbial biofilms. Journal of Bacteriology. ,vol. 173, pp. 6558- 6567 ,(1991) , 10.1128/JB.173.20.6558-6567.1991
Alex T. Nielsen, Tim Tolker-Nielsen, Kim B. Barken, Soren Molin, Role of commensal relationships on the spatial structure of a surface-attached microbial consortium Environmental Microbiology. ,vol. 2, pp. 59- 68 ,(2000) , 10.1046/J.1462-2920.2000.00084.X
J. D. Bryers, J. Robbins, J. W. Costerton, W. F. McCoy, Observations of fouling biofilm formation. Canadian Journal of Microbiology. ,vol. 27, pp. 910- 917 ,(1981) , 10.1139/M81-143
Paul Stoodley, Zbigniew Lewandowski, John D. Boyle, Hilary M. Lappin-Scott, The formation of migratory ripples in a mixed species bacterial biofilm growing in turbulent flow Environmental Microbiology. ,vol. 1, pp. 447- 455 ,(1999) , 10.1046/J.1462-2920.1999.00055.X
Sünje Johanna Pamp, Claus Sternberg, Tim Tolker-Nielsen, Insight into the microbial multicellular lifestyle via flow‐cell technology and confocal microscopy Cytometry Part A. ,vol. 75, pp. 90- 103 ,(2009) , 10.1002/CYTO.A.20685
Xinmin Yang, Haluk Beyenal, Gary Harkin, Zbigniew Lewandowski, Quantifying biofilm structure using image analysis Journal of Microbiological Methods. ,vol. 39, pp. 109- 119 ,(2000) , 10.1016/S0167-7012(99)00097-4
J C Nickel, I Ruseska, J B Wright, J W Costerton, Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrobial Agents and Chemotherapy. ,vol. 27, pp. 619- 624 ,(1985) , 10.1128/AAC.27.4.619