Transforming growth factor β inhibits steroidogenic acute regulatory (StAR) protein expression in human ovarian thecal cells
作者： George R Attia , Christina A Dooley , William E Rainey , Bruce R Carr
DOI: 10.1016/S0303-7207(00)00335-X
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摘要: Abstract In this study, we investigated the effects of TGFβ1 on steroidogensis and expression steroidogenic acute regulatory (StAR) protein which regulates an important early step in pathway. We utilized a human ovarian thecal like tumor (HOTT) cell model activin-A, inhibin-A, or presence forskolin effect dibutyryl cyclic AMP (dbcAMP) steroid accumulation culture medium. Cells were also treated with different concentration forskolin, combined production was measured at end 48 h after 3 incubation 22R-hydroxycholesterol. there dose-dependent inhibition androstenedione production. Inhibition apparent highest tested. 22R-hydroxycholesterol, significantly inhibited lower concentrations. StAR dependent manner. There similar mRNA. These results suggest that possibly follicular development may be part due to its expression.
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