Sensitization of ovarian cancer cells to cisplatin by genistein: the role of NF-kappaB

摘要: Platinum-resistance (PR) continues to be a major problem in the management of epithelial ovarian cancer (EOC). Response various chemotherapeutic agents is poor patients deemed PR. Genistein, soy isoflavone has been shown enhance effect chemotherapy prostate and pancreatic cells vitro vivo by reversing chemo-resistance phenotype. The goal this study was investigate effects combination therapy with genistein cisplatin as well other cytotoxic conventional platinum-sensitive (PS) resistant EOC cells. PS human cell line A2780 its PR clone C200 were pretreated genistein, followed either cisplatin, taxotere or gemcitabine. Cell survival apoptosis assessed MTT histone-DNA ELISA. Electrophoretic mobility shift assay (EMSA) used evaluate NF-κB DNA binding activity. Western blot analysis performed antibodies Bcl-2, Bcl-xL, survivin, c-IAP PARP. Reduction viability, corresponding induction observed pretreatment treatment each drugs both lines. for 24 hours; contrast, required 48 hours achieve response. anti-apoptotic genes c-IAP1, survivin activity all found down-regulated groups. This convincingly demonstrated that current strategy can translated pre-clinical animal model, thus it should stimulate future clinical trial drug-resistant cancer.